Total magnification achieved through a light microscope is determined by multiplying the magnifying power of the objective lens with the magnifying power of the eyepiece lens. For example, an objective lens with a 40x magnification used in conjunction with an eyepiece lens of 10x magnification yields a total magnification of 400x (40 multiplied by 10). This resulting value indicates how much larger the viewed image appears compared to the actual specimen.
Understanding the resultant image enlargement afforded by a microscope setup is essential for accurate observation and measurement of microscopic specimens. Precise magnification knowledge allows researchers and technicians to correctly interpret the size and structural details of observed objects, contributing to advancements in fields like biology, medicine, and materials science. Historically, accurate assessment of the magnified image has been a cornerstone in identifying cellular structures, pathogens, and material compositions.