This analytical tool facilitates the conversion of chromatographic methods from High-Performance Liquid Chromatography (HPLC) to Ultra-Performance Liquid Chromatography (UPLC). It calculates adjusted parameters, such as flow rate, gradient times, and injection volumes, to maintain separation performance when switching between systems with different column dimensions and particle sizes. For example, if an HPLC method uses a 4.6 mm x 150 mm column with 5 m particles, the tool assists in determining equivalent conditions for a UPLC system employing a 2.1 mm x 100 mm column with 1.7 m particles.
The application of this type of tool streamlines method redevelopment, saving time and resources. Historically, method transfer involved manual calculations and empirical adjustments, prone to error and requiring extensive experimentation. The calculator reduces this burden, allowing analysts to leverage the advantages of UPLC, such as faster run times and increased resolution, while preserving the integrity of validated HPLC methods. This is especially relevant in regulated industries where method modifications must be thoroughly documented and justified.
